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Regression testing involves retesting software to ensure that changes, such as modifications, enhancements, or bug fixes, have not introduced new issues or caused existing functionality to break. It is crucial in acceptance testing to maintain the quality and stability of software systems. This type of testing helps validate that previous tests continue to pass after updates have been made, ensuring consistent performance across iterations. By continually verifying that the system behaves as expected, regression testing instills confidence in the reliability and robustness of the software throughout its lifecycle.