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RNA Interference, or RNAi, is a cellular process that uses small RNA molecules to silence gene expression post-transcriptionally. It plays a crucial role in regulating genetic networks and defending against invasive nucleic acids such as transposons and viruses. In the context of Genetic Engineering, RNAi offers a powerful tool for targeted gene knockdown, enabling functional genomics studies and therapeutic applications by selectively suppressing specific gene products. This mechanism is an integral part of the Genetics field, contributing to our understanding of gene regulation and its impact on various biological processes within Science.